File format:
The following is the list of columns required for "Reference Gel Data" page.
Column Heading | What to store in each column | Column - Required or Optional |
---|---|---|
Spot_id | Reference Spot Number | Required |
Plate | Plate number | Optional |
Well | Well number | Optional |
Annotation | Annotation for the protein | Required |
Genome_Link | Unique ID of the Genbank accession number | Optional |
Score | score hit | Optional |
Coverage | Percent coverage | Optional |
Peptide_Matched | Number of peptides matched | Optional |
pI | pI of the matched protein | Optional |
MW | MW of the matched protein | Optional |
Gel_pI | pI of the reference spot | Required |
Gel_MW | MW of the reference spot | Required |
Support | Matched Protein or TC | Required |
Class | Biological process the protein belongs to - e.g. primary metabolism | Optional |
The column headers must have the same name as the ones in the table above. The order of the columns are not neccesarily the same as the above table. Annotation field must not have unknown proteins named as just "unknown" but should follow a naming scheme as explained by the following example: JS-2D-P1-00011971600-04770
where:- JS --- represent the initials for the John Smith lab that generated the data.
- 2D --- specifies that the data is generated using 2D-PAGE.
- P1 --- represents "Protocol 1" used for the protein sample.
- 00119716 --- represents the Mol. Wt. of the spot = 11971.6 Da. The format is #######.#.
- 04770 --- represents the pI of the spot = 4.77. The format is ##.###.