File format:
Each technical replicate for each biological sample will have a separate file. The metadata will be specified at the time of uploading the file through the front end. The file should be tab-delimited and should have the following columns:
Column Heading | What to store in each column | Requirement (Required or Optional) |
---|---|---|
Spot_id | ID for the reference spot | Required |
pI | pI for the spot on the sample gel | Optional |
MW | MW for the spot on the sample gel | Optional |
Intensity | Intensity of the spot | Optional |
Norm_intensity | Normalized intensity of the spot | Required |
At least one of the values of "Intensity" or "Norm_intensity" must be not NULL.
File names:
Please name your files using only the following characters “a–z”, “A–Z”, “0–9” and “_”. Connect parts of the name with underscores. Please have the same name for raw data and processed data files for the same sample. For example, xxx_yy_zzz.txt and xxx_yy_zzz.TIF correspond to one sample for which xxx_yy_zzz.TIF is the raw data file and xxx_yy_zzz.txt is the processed data file.
Here is an example of the format for processed proteomics data file:Spot_id | pI | MW | Intensity | Norm_intensity |
---|---|---|---|---|
89 | 6.02 | 105024 |   | 0.137 |
91 | 4.76 | 104772 |   | 0.008 |
92 | 4.81 | 104772 |   | 0.103 |
95 | 6.08 | 104646 |   | 0.024 |
97 | 5.25 | 104145 |   | 0.007 |
... | ... | ... | .. | ... |